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Structure and expression profile of the phosphate Pht1 transporter gene family in mycorrhizal Populus trichocarpa.

Identifieur interne : 002D01 ( Main/Exploration ); précédent : 002D00; suivant : 002D02

Structure and expression profile of the phosphate Pht1 transporter gene family in mycorrhizal Populus trichocarpa.

Auteurs : Ver Nica Loth-Pereda [France] ; Elena Orsini ; Pierre-Emmanuel Courty ; Frédéric Lota ; Annegret Kohler ; Loic Diss ; Damien Blaudez ; Michel Chalot ; Uwe Nehls ; Marcel Bucher ; Francis Martin

Source :

RBID : pubmed:21705655

Descripteurs français

English descriptors

Abstract

Gene networks involved in inorganic phosphate (Pi) acquisition and homeostasis in woody perennial species able to form mycorrhizal symbioses are poorly known. Here, we describe the features of the 12 genes coding for Pi transporters of the Pht1 family in poplar (Populus trichocarpa). Individual Pht1 transporters play distinct roles in acquiring and translocating Pi in different tissues of mycorrhizal and nonmycorrhizal poplar during different growth conditions and developmental stages. Pi starvation triggered the up-regulation of most members of the Pht1 family, especially PtPT9 and PtPT11. PtPT9 and PtPT12 showed a striking up-regulation in ectomycorrhizas and endomycorrhizas, whereas PtPT1 and PtPT11 were strongly down-regulated. PtPT10 transcripts were highly abundant in arbuscular mycorrhiza (AM) roots only. PtPT8 and PtPT10 are phylogenetically associated to the AM-inducible Pht1 subfamily I. The analysis of promoter sequences revealed conserved motifs similar to other AM-inducible orthologs in PtPT10 only. To gain more insight into gene regulatory mechanisms governing the AM symbiosis in woody plant species, the activation of the poplar PtPT10 promoter was investigated and detected in AM of potato (Solanum tuberosum) roots. These results indicated that the regulation of AM-inducible Pi transporter genes is conserved between perennial woody and herbaceous plant species. Moreover, poplar has developed an alternative Pi uptake pathway distinct from AM plants, allowing ectomycorrhizal poplar to recruit PtPT9 and PtPT12 to cope with limiting Pi concentrations in forest soils.

DOI: 10.1104/pp.111.180646
PubMed: 21705655
PubMed Central: PMC3149965


Affiliations:


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Le document en format XML

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<term>Gene Expression Regulation, Plant (drug effects)</term>
<term>Genes, Plant (genetics)</term>
<term>Genotype (MeSH)</term>
<term>Glomeromycota (drug effects)</term>
<term>Glomeromycota (growth & development)</term>
<term>Glomeromycota (physiology)</term>
<term>Glucuronidase (metabolism)</term>
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<term>Multigene Family (genetics)</term>
<term>Mycorrhizae (drug effects)</term>
<term>Mycorrhizae (physiology)</term>
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<term>Phosphate Transport Proteins (genetics)</term>
<term>Phosphate Transport Proteins (metabolism)</term>
<term>Phosphates (pharmacology)</term>
<term>Phylogeny (MeSH)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (drug effects)</term>
<term>Populus (genetics)</term>
<term>Populus (microbiology)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Species Specificity (MeSH)</term>
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<term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Annotation de séquence moléculaire (MeSH)</term>
<term>Famille multigénique (génétique)</term>
<term>Glomeromycota (croissance et développement)</term>
<term>Glomeromycota (effets des médicaments et des substances chimiques)</term>
<term>Glomeromycota (physiologie)</term>
<term>Glucuronidase (métabolisme)</term>
<term>Gènes de plante (génétique)</term>
<term>Génotype (MeSH)</term>
<term>Mycorhizes (effets des médicaments et des substances chimiques)</term>
<term>Mycorhizes (physiologie)</term>
<term>Numération de colonies microbiennes (MeSH)</term>
<term>Phosphates (pharmacologie)</term>
<term>Phylogenèse (MeSH)</term>
<term>Populus (effets des médicaments et des substances chimiques)</term>
<term>Populus (génétique)</term>
<term>Populus (microbiologie)</term>
<term>Protéines de transport du phosphate (composition chimique)</term>
<term>Protéines de transport du phosphate (génétique)</term>
<term>Protéines de transport du phosphate (métabolisme)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term>
<term>Spécificité d'espèce (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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<term>Phosphate Transport Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Phosphate Transport Proteins</term>
<term>RNA, Messenger</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en">
<term>Glucuronidase</term>
<term>Phosphate Transport Proteins</term>
<term>RNA, Messenger</term>
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<term>Protéines de transport du phosphate</term>
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<term>Glomeromycota</term>
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<term>Populus</term>
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<term>Glomeromycota</term>
<term>Mycorhizes</term>
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<term>Régulation de l'expression des gènes végétaux</term>
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<term>Genes, Plant</term>
<term>Multigene Family</term>
<term>Populus</term>
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<term>Glomeromycota</term>
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<term>ARN messager</term>
<term>Famille multigénique</term>
<term>Gènes de plante</term>
<term>Populus</term>
<term>Protéines de transport du phosphate</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr">
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en">
<term>Populus</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>ARN messager</term>
<term>Glucuronidase</term>
<term>Protéines de transport du phosphate</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr">
<term>Phosphates</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en">
<term>Phosphates</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr">
<term>Glomeromycota</term>
<term>Mycorhizes</term>
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<term>Gene Expression Profiling</term>
<term>Genotype</term>
<term>Molecular Sequence Annotation</term>
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<term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<term>Analyse de profil d'expression de gènes</term>
<term>Annotation de séquence moléculaire</term>
<term>Génotype</term>
<term>Numération de colonies microbiennes</term>
<term>Phylogenèse</term>
<term>RT-PCR</term>
<term>Spécificité d'espèce</term>
<term>Végétaux génétiquement modifiés</term>
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<div type="abstract" xml:lang="en">Gene networks involved in inorganic phosphate (Pi) acquisition and homeostasis in woody perennial species able to form mycorrhizal symbioses are poorly known. Here, we describe the features of the 12 genes coding for Pi transporters of the Pht1 family in poplar (Populus trichocarpa). Individual Pht1 transporters play distinct roles in acquiring and translocating Pi in different tissues of mycorrhizal and nonmycorrhizal poplar during different growth conditions and developmental stages. Pi starvation triggered the up-regulation of most members of the Pht1 family, especially PtPT9 and PtPT11. PtPT9 and PtPT12 showed a striking up-regulation in ectomycorrhizas and endomycorrhizas, whereas PtPT1 and PtPT11 were strongly down-regulated. PtPT10 transcripts were highly abundant in arbuscular mycorrhiza (AM) roots only. PtPT8 and PtPT10 are phylogenetically associated to the AM-inducible Pht1 subfamily I. The analysis of promoter sequences revealed conserved motifs similar to other AM-inducible orthologs in PtPT10 only. To gain more insight into gene regulatory mechanisms governing the AM symbiosis in woody plant species, the activation of the poplar PtPT10 promoter was investigated and detected in AM of potato (Solanum tuberosum) roots. These results indicated that the regulation of AM-inducible Pi transporter genes is conserved between perennial woody and herbaceous plant species. Moreover, poplar has developed an alternative Pi uptake pathway distinct from AM plants, allowing ectomycorrhizal poplar to recruit PtPT9 and PtPT12 to cope with limiting Pi concentrations in forest soils.</div>
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